Load all required libraries.

library(tidyverse)
## -- Attaching packages --------------------------------------- tidyverse 1.3.1 --
## v ggplot2 3.3.5     v purrr   0.3.4
## v tibble  3.1.3     v dplyr   1.0.7
## v tidyr   1.1.3     v stringr 1.4.0
## v readr   2.0.0     v forcats 0.5.1
## -- Conflicts ------------------------------------------ tidyverse_conflicts() --
## x dplyr::filter() masks stats::filter()
## x dplyr::lag()    masks stats::lag()
library(plotly)
## 
## Attaching package: 'plotly'
## The following object is masked from 'package:ggplot2':
## 
##     last_plot
## The following object is masked from 'package:stats':
## 
##     filter
## The following object is masked from 'package:graphics':
## 
##     layout
library(broom)

Read in raw data from RDS.

raw_data <- readRDS("./year2.RDS")

Make a few small modifications to names and data for visualizations.

final_data <- raw_data %>% mutate(log_copy_per_L = log10(mean_copy_num_L)) %>%
  rename(Facility = wrf) %>%
  mutate(Facility = recode(Facility, 
                           "NO" = "WRF A",
                           "MI" = "WRF B",
                           "CC" = "WRF C"))

Seperate the data by gene target to ease layering in the final plot

#make three data layers
only_positives <<- subset(final_data, (!is.na(final_data$Facility)))
only_n1 <- subset(only_positives, target == "N1")
only_n2 <- subset(only_positives, target == "N2")
only_background <<-final_data %>% 
  select(c(date, cases_cum_clarke, new_cases_clarke, X7_day_ave_clarke)) %>%
  group_by(date) %>% summarise_if(is.numeric, mean)

#specify fun colors
background_color <- "#7570B3"
seven_day_ave_color <- "#E6AB02"
marker_colors <- c("N1" = '#1B9E77',"N2" ='#D95F02')
#remove facilty C for now
#only_n1 <- only_n1[!(only_n1$Facility == "WRF C"),]
#only_n2 <- only_n2[!(only_n2$Facility == "WRF C"),]

only_n1 <- only_n1[!(only_n1$Facility == "WRF A" & only_n1$date == "2020-11-02"), ]
only_n2 <- only_n2[!(only_n2$Facility == "WRF A" & only_n2$date == "2020-11-02"), ]

Build the main plot

      #first layer is the background epidemic curve
        p1 <- only_background %>%
              plotly::plot_ly() %>%
              plotly::add_trace(x = ~date, y = ~new_cases_clarke, 
                                type = "bar", 
                                hoverinfo = "text",
                                text = ~paste('</br> Date: ', date,
                                                     '</br> Daily Cases: ', new_cases_clarke),
                                alpha = 0.5,
                                name = "Daily Reported Cases",
                                color = background_color,
                                colors = background_color,
                                showlegend = FALSE) %>%
            layout(yaxis = list(title = "Clarke County Daily Cases", showline=TRUE)) %>%
            layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
        
        #renders the main plot layer two as seven day moving average
        p1 <- p1 %>% plotly::add_trace(x = ~date, y = ~X7_day_ave_clarke, 
                             type = "scatter",
                             mode = "lines",
                             hoverinfo = "text",
                            text = ~paste('</br> Date: ', date,
                                                     '</br> Seven-Day Moving Average: ', X7_day_ave_clarke),
                             name = "Seven Day Moving Average Athens",
                             line = list(color = seven_day_ave_color),
                             showlegend = FALSE)
      

        
        #renders the main plot layer three as positive target hits
        
        p2 <- plotly::plot_ly() %>%
          plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
                                       type = "scatter",
                                       mode = "markers",
                                       hoverinfo = "text",
                                       text = ~paste('</br> Date: ', date,
                                                     '</br> Facility: ', Facility,
                                                     '</br> Target: ', target,
                                                     '</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
                                       data = only_n1,
                                       symbol = ~Facility,
                                       marker = list(color = '#1B9E77', size = 8, opacity = 0.65),
                                       showlegend = FALSE) %>%
          plotly::add_trace(x = ~date, y = ~mean_copy_num_L,
                                       type = "scatter",
                                       mode = "markers",
                                       hoverinfo = "text",
                                       text = ~paste('</br> Date: ', date,
                                                     '</br> Facility: ', Facility,
                                                     '</br> Target: ', target,
                                                     '</br> Copies/L: ', round(mean_copy_num_L, digits = 2)),
                                       data = only_n2,
                                       symbol = ~Facility,
                                       marker = list(color = '#D95F02', size = 8, opacity = 0.65),
                                       showlegend = FALSE) %>%
            layout(yaxis = list(title = "SARS CoV-2 Copies/L", 
                                 showline = TRUE,
                                 type = "log",
                                 dtick = 1,
                                 automargin = TRUE)) %>%
            layout(legend = list(orientation = "h", x = 0.2, y = -0.3))
        
        #adds the limit of detection dashed line
        p2 <- p2 %>% plotly::add_segments(x = as.Date("2021-06-30"), 
                                          xend = ~max(date + 10), 
                                          y = 3571.429, yend = 3571.429,
                                          opacity = 0.35,
                                          line = list(color = "black", dash = "dash")) %>%
          layout(annotations = list(x = as.Date("2021-06-30"), y = 3.8, xref = "x", yref = "y", 
                                    text = "Limit of Detection", showarrow = FALSE))

        

        p1
        p2

Combine the two main plot pieces as a subplot

#seperate n1 and n2 frames by site
#n1
wrf_a_only_n1 <- subset(only_n1, Facility == "WRF A")
wrf_b_only_n1 <- subset(only_n1, Facility == "WRF B")
wrf_c_only_n1 <- subset(only_n1, Facility == "WRF C")

#n2
wrf_a_only_n2 <- subset(only_n2, Facility == "WRF A")
wrf_b_only_n2 <- subset(only_n2, Facility == "WRF B")
wrf_c_only_n2 <- subset(only_n2, Facility == "WRF C")


#rejoin the old data frames then seperate in to averages for each plant. 
wrfa_both <- full_join(wrf_a_only_n1, wrf_a_only_n2)%>%
  select(c(date, mean_total_copies)) %>%
  group_by(date) %>%
  summarize_if(is.numeric, mean) %>%
  ungroup() %>%
  mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfb_both <- full_join(wrf_b_only_n1, wrf_b_only_n2)%>%
  select(c(date, mean_total_copies)) %>%
  group_by(date) %>%
  summarize_if(is.numeric, mean) %>%
  ungroup() %>%
  mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
wrfc_both <- full_join(wrf_c_only_n1, wrf_c_only_n2)%>%
  select(c(date, mean_total_copies)) %>%
  group_by(date) %>%
  summarize_if(is.numeric, mean) %>%
  ungroup() %>%
  mutate(log_total_copies_both = log10(mean_total_copies))
## Joining, by = c("date", "new_cases_clarke", "cases_cum_clarke", "X7_day_ave_clarke", "Facility", "collection_num", "target", "mean_copy_num_uL_rxn", "mean_copy_num_L", "sd_L", "mean_total_copies", "sd_total_copies", "log_copy_per_L")
#get max date
maxdate <- max(wrfa_both$date)
mindate <- min(wrfa_both$date)

Build loess smoothing figures figures

This makes the individual plots

#**************************************WRF A PLOT**********************************************
#add trendlines 
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_botha <- ggplot(wrfa_both, aes(x = date, y = log_total_copies_both)) + 
  stat_smooth(aes(outfit=fit_botha<<-..y..), method = "loess", color = '#1B9E77', 
              span = 0.6, n = 71)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_botha
## `geom_smooth()` using formula 'y ~ x'

fit_botha
##  [1] 11.55318 11.58589 11.61880 11.65190 11.68520 11.71869 11.75237 11.78618
##  [9] 11.82008 11.85410 11.88829 11.92268 11.95730 11.99323 12.03104 12.07001
## [17] 12.10943 12.14859 12.18675 12.22321 12.25702 12.28819 12.31747 12.34561
## [25] 12.37336 12.40146 12.43067 12.46905 12.50704 12.53542 12.56229 12.58793
## [33] 12.61261 12.63659 12.66016 12.68358 12.70521 12.72408 12.74155 12.75893
## [41] 12.77755 12.79876 12.82386 12.85489 12.89065 12.92770 12.96259 12.99186
## [49] 13.02059 13.05439 13.09067 13.12679 13.16015 13.18814 13.20812 13.22185
## [57] 13.23266 13.24031 13.24460 13.24530 13.24220 13.23506 13.22419 13.20976
## [65] 13.19158 13.16982 13.14451 13.11565 13.08327 13.04738 13.00802
#assign fits to a vector
both_trenda <- fit_botha

#extract y min and max for each
limits_botha <- ggplot_build(extract_botha)$data
## `geom_smooth()` using formula 'y ~ x'
limits_botha <- as.data.frame(limits_botha)
both_ymina <- limits_botha$ymin
both_ymaxa <- limits_botha$ymax

#reassign dataframes (just to be safe)
work_botha <- wrfa_both

#fill in missing dates to smooth fits
work_botha <- work_botha %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_botha <- work_botha$date

#create a new smooth dataframe to layer
smooth_frame_botha <- data.frame(date_vec_botha, both_trenda, both_ymina, both_ymaxa)
#WRF A
#plot smooth frames
p_wrf_a <- plotly::plot_ly() %>%
  plotly::add_lines(x = ~date_vec_botha, y = ~both_trenda,
                    data = smooth_frame_botha,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_botha,
                                  '</br> Median Log Copies: ', round(both_trenda, digits = 2)),
                    line = list(color = '#1B9E77', size = 8, opacity = 0.65),
                    showlegend = FALSE) %>%
     layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_botha, ymin = ~both_ymina, ymax = ~both_ymaxa,
                    showlegend = FALSE,
                    opacity = 0.25,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_botha, #leaving in case we want to change
                                  '</br> Max Log Copies: ', round(both_ymaxa, digits = 2),
                                  '</br> Min Log Copies: ', round(both_ymina, digits = 2)),
                    name = "",
                    fillcolor = '#1B9E77',
                    line = list(color = '#1B9E77')) %>%
                layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies", 
                                 showline = TRUE,
                                 automargin = TRUE)) %>%
                layout(xaxis = list(title = "Date")) %>%
                layout(title = "WRF A") %>%
  plotly::add_markers(x = ~date, y = ~log_total_copies_both,
                      data = wrfa_both,
                       hoverinfo = "text",
                       showlegend = FALSE,
                       text = ~paste('</br> Date: ', date, 
                                     '</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
                       marker = list(color = '#1B9E77', size = 6, opacity = 0.65))

p_wrf_a
save(p_wrf_a, file = "./site_objects/wrf_a_year2.rda")
#**************************************WRF B PLOT**********************************************
#add trendlines 
#extract data from geom_smooth
#both extract
# *********************************span 0.6***********************************
#*****************Must always update the n = TOTAL NUMBER OF DAYS*************************
extract_bothb <- ggplot(wrfb_both, aes(x = date, y = log_total_copies_both)) + 
  stat_smooth(aes(outfit=fit_bothb<<-..y..), method = "loess", color = '#D95F02', 
              span = 0.6, n = 71)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothb
## `geom_smooth()` using formula 'y ~ x'

fit_bothb
##  [1] 10.72752 10.81467 10.90026 10.98427 11.06668 11.14746 11.22661 11.30413
##  [9] 11.38009 11.45456 11.52760 11.59930 11.66970 11.73767 11.80248 11.86480
## [17] 11.92532 11.98473 12.04370 12.10294 12.16667 12.23662 12.30973 12.38291
## [25] 12.45309 12.51720 12.57215 12.62697 12.67835 12.71797 12.75237 12.78221
## [33] 12.80817 12.83091 12.85112 12.86946 12.88030 12.88019 12.87307 12.86287
## [41] 12.85354 12.84903 12.85326 12.86484 12.87868 12.89326 12.90703 12.91844
## [49] 12.93090 12.94757 12.96652 12.98581 13.00351 13.01769 13.02640 13.03095
## [57] 13.03385 13.03498 13.03425 13.03153 13.02673 13.01974 13.01059 12.99945
## [65] 12.98633 12.97123 12.95415 12.93512 12.91415 12.89127 12.86650
#assign fits to a vector
both_trendb <- fit_bothb

#extract y min and max for each
limits_bothb <- ggplot_build(extract_bothb)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothb <- as.data.frame(limits_bothb)
both_yminb <- limits_bothb$ymin
both_ymaxb <- limits_bothb$ymax

#reassign dataframes (just to be safe)
work_bothb <- wrfb_both

#fill in missing dates to smooth fits
work_bothb <- work_bothb %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothb <- work_bothb$date

#create a new smooth dataframe to layer
smooth_frame_bothb <- data.frame(date_vec_bothb, both_trendb, both_yminb, both_ymaxb)
#WRF B
#plot smooth frames
p_wrf_b <- plotly::plot_ly() %>%
  plotly::add_lines(x = ~date_vec_bothb, y = ~both_trendb,
                    data = smooth_frame_bothb,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_bothb,
                                  '</br> Median Log Copies: ', round(both_trendb, digits = 2)),
                    line = list(color = '#D95F02', size = 8, opacity = 0.65),
                    showlegend = FALSE) %>%
     layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothb, ymin = ~both_yminb, ymax = ~both_ymaxb,
                    showlegend = FALSE,
                    opacity = 0.25,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_bothb, #leaving in case we want to change
                                  '</br> Max Log Copies: ', round(both_ymaxb, digits = 2),
                                  '</br> Min Log Copies: ', round(both_yminb, digits = 2)),
                    name = "",
                    fillcolor = '#D95F02',
                    line = list(color = '#D95F02')) %>%
                layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies", 
                                 showline = TRUE,
                                 automargin = TRUE)) %>%
                layout(xaxis = list(title = "Date")) %>%
                layout(title = "WRF B") %>%
  plotly::add_markers(x = ~date, y = ~log_total_copies_both,
                      data = wrfb_both,
                       hoverinfo = "text",
                       showlegend = FALSE,
                       text = ~paste('</br> Date: ', date, 
                                     '</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
                       marker = list(color = '#D95F02', size = 6, opacity = 0.65))

p_wrf_b
save(p_wrf_b, file = "./site_objects/wrf_b_year2.rda")

#**************************************WRF C PLOT********************************************** #add trendlines #extract data from geom_smooth # *********************************span 0.6*********************************** #*****************Must always update the n = TOTAL NUMBER OF DAYS*************************

extract_bothc <- ggplot(wrfc_both, aes(x = date, y = log_total_copies_both)) + 
  stat_smooth(aes(outfit=fit_bothc<<-..y..), method = "loess", color = '#E7298A', 
              span = 0.6, n = 71)
## Warning: Ignoring unknown aesthetics: outfit
#look at the fits to align dates and total observations
#both
extract_bothc
## `geom_smooth()` using formula 'y ~ x'

fit_bothc
##  [1] 10.62239 10.73383 10.84063 10.94276 11.04021 11.13295 11.22095 11.30429
##  [9] 11.38305 11.45722 11.52680 11.59176 11.65212 11.70413 11.74569 11.77923
## [17] 11.80719 11.83197 11.85603 11.88178 11.90744 11.93039 11.95178 11.97277
## [25] 11.99454 12.01822 12.04499 12.06191 12.07840 12.10708 12.13694 12.16721
## [33] 12.19711 12.22584 12.25265 12.27673 12.30104 12.32821 12.35697 12.38611
## [41] 12.41437 12.44051 12.46328 12.47939 12.48874 12.49495 12.50162 12.51235
## [49] 12.52512 12.53598 12.54560 12.55468 12.56390 12.57394 12.58548 12.59928
## [57] 12.61503 12.63178 12.64859 12.66452 12.67862 12.68996 12.70091 12.71104
## [65] 12.71857 12.72516 12.73076 12.73532 12.73881 12.74117 12.74237
#assign fits to a vector
both_trendc <- fit_bothc

#extract y min and max for each
limits_bothc <- ggplot_build(extract_bothc)$data
## `geom_smooth()` using formula 'y ~ x'
limits_bothc <- as.data.frame(limits_bothc)
both_yminc <- limits_bothc$ymin
both_ymaxc <- limits_bothc$ymax

#reassign dataframes (just to be safe)
work_bothc <- wrfc_both

#fill in missing dates to smooth fits
work_bothc <- work_bothc %>% complete(date = seq(min(date), max(date), by = "1 day"))
date_vec_bothc <- work_bothc$date

#create a new smooth dataframe to layer
smooth_frame_bothc <- data.frame(date_vec_bothc, both_trendc, both_yminc, both_ymaxc)
#WRF C
#plot smooth frames
p_wrf_c <- plotly::plot_ly() %>%
  plotly::add_lines(x = ~date_vec_bothc, y = ~both_trendc,
                    data = smooth_frame_bothc,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_bothc,
                                  '</br> Median Log Copies: ', round(both_trendc, digits = 2)),
                    line = list(color = '#E7298A', size = 8, opacity = 0.65),
                    showlegend = FALSE) %>%
     layout(xaxis = list(range = c(mindate - 7, maxdate + 7))) %>% #buffer here
plotly::add_ribbons(x ~date_vec_bothc, ymin = ~both_yminc, ymax = ~both_ymaxc,
                    showlegend = FALSE,
                    opacity = 0.25,
                    hoverinfo = "text",
                    text = ~paste('</br> Date: ', date_vec_bothc, #leaving in case we want to change
                                  '</br> Max Log Copies: ', round(both_ymaxc, digits = 2),
                                  '</br> Min Log Copies: ', round(both_yminc, digits = 2)),
                    name = "",
                    fillcolor = '#E7298A',
                    line = list(color = '#E7298A')) %>%
                layout(yaxis = list(title = "Total Log10 SARS CoV-2 Copies", 
                                 showline = TRUE,
                                 automargin = TRUE)) %>%
                layout(xaxis = list(title = "Date")) %>%
                layout(title = "WRF C") %>%
  plotly::add_markers(x = ~date, y = ~log_total_copies_both,
                      data = wrfc_both,
                       hoverinfo = "text",
                       showlegend = FALSE,
                       text = ~paste('</br> Date: ', date, 
                                     '</br> Actual Log Copies: ', round(log_total_copies_both, digits = 2)),
                       marker = list(color = '#E7298A', size = 6, opacity = 0.65))

p_wrf_c
save(p_wrf_c, file = "./site_objects/wrf_c_year2.rda")

keeping in case

#save(wrfa_both, file = "./plotly_objs/wrfa_both.rda")
#save(wrfb_both, file = "./plotly_objs/wrfb_both.rda")
#save(wrfc_both, file = "./plotly_objs/wrfc_both.rda")
#save(date_vec_botha, file = "./plotly_objs/date_vec_botha.rda")
#save(date_vec_bothb, file = "./plotly_objs/date_vec_bothb.rda")
#save(date_vec_bothc, file = "./plotly_objs/date_vec_bothc.rda")
#save(both_ymina, file = "./plotly_objs/both_ymina.rda")
#save(both_ymaxa, file = "./plotly_objs/both_ymaxa.rda")

#save(both_yminb, file = "./plotly_objs/both_yminb.rda")
#save(both_ymaxb, file = "./plotly_objs/both_ymaxb.rda")

#save(both_yminc, file = "./plotly_objs/both_yminc.rda")
#save(both_ymaxc, file = "./plotly_objs/both_ymaxc.rda")